Recombinant Human CD27/TNFRSF7 Protein (Fc & His Tag)

Recombinant Human CD27/TNFRSF7 Protein (Fc & His Tag)

SKU #	PKSH032210
Expression Host	HEK293 Cells

Description

Synonyms	S152,S152. LPFS2,S152.LPFS2,T14,TNFRSF7,Tp55
Species	Human
Expression Host	HEK293 Cells
Sequence	Thr 21-Ile192
Accession	P26842
Calculated Molecular Weight	47.2 kDa
Observed Molecular Weight	60 kDa
Tag	C-Fc-His
Bio-activity	Not validated for activity

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 m filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background
CD27 antigen is also known as CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; T14 and TNFRSF7. In humans; it is encoded by the CD27 gene. CD27 is a single-pass type I membrane protein with 3 TNFR-Cys repeats. It is a member of the TNF-receptor superfamily and is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70; and plays a key role in regulating B-cell activation and immunoglobulin synthesis. It plays a role in survival of activated T-cells and apoptosis through association with SIVA1.